Regulatory

Part:BBa_K2549060:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-13)


minimal CMV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the binding sites increase, the transcription process is effected unpleasantly due to the steric effect. Thus we choose a modified version of CMV promotor which is more sensitive to transcription regulation and less effected by the increase of binding sites.


Source

From IDT (gBlock), human codon optimized